Flow cytometry and microscopic acridine orange test: relationship with standard semen analysis.

@article{Apedaile2004FlowCA,
  title={Flow cytometry and microscopic acridine orange test: relationship with standard semen analysis.},
  author={Anwyn E Apedaile and Charles D. Garrett and De Yi Liu and Gary N. Clarke and Stephanie A Johnston and Hugh William Gordon Baker},
  journal={Reproductive biomedicine online},
  year={2004},
  volume={8 4},
  pages={
          398-407
        }
}
Improved prediction of male fertility requires advances in semen analysis. This study examined the reproducibility and independence of the flow cytometry acridine orange test (FCM-AOT) of sperm chromatin integrity as an assessment of semen quality. The study found that FCM-AOT results are not significantly affected by up to 6 h delay in semen preparation (n = 9) or contamination of semen with moderate concentrations of bacteria (<10(8)/ml E. coli or Staph. epidermidis, n = 14). The variation of… CONTINUE READING
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