Flow cytometry: principles and clinical applications in hematology.

@article{Brown2000FlowCP,
  title={Flow cytometry: principles and clinical applications in hematology.},
  author={M Brown and Carl T. Wittwer},
  journal={Clinical chemistry},
  year={2000},
  volume={46 8 Pt 2},
  pages={
          1221-9
        }
}
The use of flow cytometry in the clinical laboratory has grown substantially in the past decade. This is attributable in part to the development of smaller, user-friendly, less-expensive instruments and a continuous increase in the number of clinical applications. Flow cytometry measures multiple characteristics of individual particles flowing in single file in a stream of fluid. Light scattering at different angles can distinguish differences in size and internal complexity, whereas light… 

Figures and Tables from this paper

Basic Theory and Clinical Applications of Flow Cytometry

Applications of FC in solid organ and hematopoeitic stem-cell transplantation, transfusion medicine, hematopathology, and infectious diseases are presented.

Principles of Flow Cytometry

This chapter is written with the view of giving a simplified overview of flow cytometry, the gating strategies and data analysis applied in diagnostic flow cytometers applied to haematological disorders, aimed at laboratory staff and junior medical staff, and even more senior staff who have not had the privilege to experience clinical flow cytometer in all its diagnostic applications.

Recent advancements of flow cytometry: new applications in hematology and oncology

Applications of flow cytometry include nucleic acid analyses in cancer cells, new methods for assessing rare circulating tumor cells and disease-specific applications in malignancy with emphasis on diagnosis and treatment of hematologic malignancies, including minimal residual disease.

Microchip-based enumeration of human white blood cells.

This protocol describes how to build a simple microfluidic cell and perform a total white blood cell count through capture and immunolabeling of the WBCs with an anti-CD45 antibody.

Flow cytometry in clinical pathology

This study reviews the application of flow cytometry within clinical pathology with an emphasis upon haematology and immunology, including the principles and pitfalls associated with leukocyte immunophenotyping for leukaemia and lymphoma diagnosis, immune deficiency and rare event detection and screening for genetic disease.

Application and Disadvantages of Flow Cytometry in Hematopathology : A Brief Review

Flow cytometry is a laser-based technology used to measure characteristics of biological particles, particularly used to perform measurements on whole cells as well as prepared cellular constituents such as nuclei and organelles.

On-chip flow cytometry: where is it now and where is it going?

With its extraordinary single-cell ana lysis and sorting capabilities, flow cytometry has been used extensively in numerous fields such as molecular biology, pathology, immunology, plant biology and marine biology.

Measurement of PTEN by Flow Cytometry.

The application of flow cytometry analysis to the tumor suppressor PTEN in various cancer cell lines and a mouse model is described.

Flow cytometry: an ‘old’ tool for novel applications in medical genetics

An overview of current and future applications is given, including the detection of viral sequences via microsphere‐based PCR assays and the analysis of single nucleotide polymorphisms, reflecting individual phenotypic traits.
...

References

SHOWING 1-10 OF 108 REFERENCES

Flow cytometry: its applications in hematology.

Four relevant hematologic fields are briefly discussed, in which FCM appears to be of great help at present: the immunophenotyping of leukemias and lymphomas, the measurement of proliferative activity and DNA ploidy in hematological malignancies, the detection of drug resistant leukemic cells and the use of FCM in the study of platelets.

Principles of flow cytometry.

  • J. Haynes
  • Biology
    Cytometry. Supplement : the journal of the Society for Analytical Cytology
  • 1988
It now remains for the research clinician to adapt flow cytometry to a broader range of useful clinical applications, as usage barely scratches the surface of the capabilities of this technology.

Flow cytometry in cytopathology. Overview and perspectives.

It is suggested that the data being provided by FCM will probably lead to a more clinically relevant classification of tumors, based more on the biochemical/molecular markers of individual cells and an assessment of tumor heterogeneity and less on classical cell morphology and tumor progression.

DNA flow cytometry in solid tumors: practical aspects and clinical applications.

The methods for sample handling, processing, and interpretation of DNA histograms are described and methodology and interpretations that have proven efficient and reproducible are presented.

DNA flow cytometry in solid tumors: practical aspects and clinical applications.

The methods for sample handling, processing, and interpretation of DNA histograms are described and methodology and interpretations that have proven efficient and reproducible are presented.

Flow cytometric analysis of thiazole orange uptake by platelets: a diagnostic aid in the evaluation of thrombocytopenic disorders.

Flow cytometric analysis of platelets after staining with TO is a sensitive and specific test that rapidly provides information on the thrombopoietic activity in thromBocytopenic disorders.

Flow cytometry in diagnosis and management of large fetomaternal haemorrhage.

Flow cytometry is helpful for the accurate quantitation and management of patients with large FMH and in cases where the presence of maternal haemoglobin F containing cells renders the Kleihauer technique inaccurate.

Multiplexed analysis of human cytokines by use of the FlowMetrix system.

The FlowMetrixTM system has been developed as a highly sensitive, multiplexed assay platform that provides a means to determine qualitative and quantitative measurements of multiple analytes simultaneously and allows the simultaneous quantification of up to 64 different cytokines or chemokines in a single tissue or fluid sample in real time.

The use of flow cytometry to measure neutrophil function.

Laboratory markers of platelet activation and their clinical significance.

Clinical applications of whole blood flow cytometric assays of platelet function in these diseases may include identification of patients who would benefit from additional antiplatelet therapy and prediction of ischemic events.
...