Flow cytometry: principles and clinical applications in hematology.

@article{Brown2000FlowCP,
  title={Flow cytometry: principles and clinical applications in hematology.},
  author={Micheal D. Brown and Carl T. Wittwer},
  journal={Clinical chemistry},
  year={2000},
  volume={46 8 Pt 2},
  pages={
          1221-9
        }
}
The use of flow cytometry in the clinical laboratory has grown substantially in the past decade. This is attributable in part to the development of smaller, user-friendly, less-expensive instruments and a continuous increase in the number of clinical applications. Flow cytometry measures multiple characteristics of individual particles flowing in single file in a stream of fluid. Light scattering at different angles can distinguish differences in size and internal complexity, whereas light… Expand
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References

SHOWING 1-10 OF 117 REFERENCES
Flow cytometry: its applications in hematology.
TLDR
Four relevant hematologic fields are briefly discussed, in which FCM appears to be of great help at present: the immunophenotyping of leukemias and lymphomas, the measurement of proliferative activity and DNA ploidy in hematological malignancies, the detection of drug resistant leukemic cells and the use of FCM in the study of platelets. Expand
Flow cytometry in cytopathology. Overview and perspectives.
TLDR
It is suggested that the data being provided by FCM will probably lead to a more clinically relevant classification of tumors, based more on the biochemical/molecular markers of individual cells and an assessment of tumor heterogeneity and less on classical cell morphology and tumor progression. Expand
Principles of flow cytometry.
TLDR
The most prominent uses of flow cytometers are for immunological characterization of lymphomas and leukemias, crossmatching tissues for organ transplants, and counting lymphocyte subpopulations in the peripheral blood of HIV-infected individuals. Expand
DNA flow cytometry in solid tumors: practical aspects and clinical applications.
TLDR
The methods for sample handling, processing, and interpretation of DNA histograms are described and methodology and interpretations that have proven efficient and reproducible are presented. Expand
DNA flow cytometry in solid tumors: practical aspects and clinical applications.
TLDR
The methods for sample handling, processing, and interpretation of DNA histograms are described and methodology and interpretations that have proven efficient and reproducible are presented. Expand
Recent advances in flow cytometry: application to the diagnosis of hematologic malignancy.
TLDR
The use of flow cytometry in the diagnosis of major disorders highlighting the prognoslymphoid differentiation approaches 98% is reviewed, although the prognostic value of immunophenotypic data is controvertically important subgroups defined either morphologically or genetically. Expand
Flow cytometric analysis of thiazole orange uptake by platelets: a diagnostic aid in the evaluation of thrombocytopenic disorders.
Thiazole orange (TO), a fluorescent dye originally synthesized for reticulocyte analysis, is characterized by a large fluorescence enhancement and high quantum yield on binding to nucleic acids,Expand
Flow cytometry in diagnosis and management of large fetomaternal haemorrhage.
TLDR
Flow cytometry is helpful for the accurate quantitation and management of patients with large FMH and in cases where the presence of maternal haemoglobin F containing cells renders the Kleihauer technique inaccurate. Expand
Clinically useful information provided by the flow cytometric immunophenotyping of hematological malignancies: current status and future directions.
TLDR
The information provided by the flow cytometric immunophenotyping of hematological malignancies is of great clinical utility, with a major challenge for the near future being the standardization of technical procedures, data interpretation, and reporting. Expand
Multiplexed analysis of human cytokines by use of the FlowMetrix system.
TLDR
The FlowMetrixTM system has been developed as a highly sensitive, multiplexed assay platform that provides a means to determine qualitative and quantitative measurements of multiple analytes simultaneously and allows the simultaneous quantification of up to 64 different cytokines or chemokines in a single tissue or fluid sample in real time. Expand
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