Flow cytometric sorting of non-human primate sperm nuclei.

  title={Flow cytometric sorting of non-human primate sperm nuclei.},
  author={Justine Kellie O'Brien and Tomas Stojanov and Scott J Heffernan and Fiona K Hollinshead and Larry Vogelnest and William Maxwell and Gareth Evans},
  volume={63 1},
Pre-determination of the sex of offspring has implications for management and conservation of captive wildlife species, particularly those with single sex-dominated social structures. Our goal is to adapt flow cytometry technology to sort spermatozoa of non-human primate species for use with assisted reproductive technologies. The objectives of this study were to: (i) determine the difference in DNA content between X- and Y-bearing spermatozoa (ii) sort sperm nuclei into X- and Y-enriched… CONTINUE READING
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