Flow cytometric screening of aldolase catalytic antibodies.


High-throughput screening of cells expressing active catalytic antibody clones by flow cytometry is described. A fluorogenic retro-aldol retro-Michael substrate was designed and synthesized with incorporation of a chloromethyl moiety for intracellular retention. Hybridoma or transfected mammalian cells expressing catalytic antibody molecules could be rapidly isolated.

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@article{Shim2004FlowCS, title={Flow cytometric screening of aldolase catalytic antibodies.}, author={Hyunbo Shim and Amelie Eriksson Karlstr{\"{o}m and Sofia M Touami and Roberta P. Fuller and Carlos F. Barbas}, journal={Bioorganic & medicinal chemistry letters}, year={2004}, volume={14 15}, pages={4065-8} }