Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine.

@article{Dolbeare1983FlowCM,
  title={Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine.},
  author={Frank Dolbeare and Howard Gratzner and Maria G. Pallavicini and Joe W. Gray},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1983},
  volume={80 18},
  pages={5573-7}
}
We have developed a procedure for simultaneous flow cytometric measurement of cellular DNA content and amount of BrdUrd incorporated into cellular DNA. Propidium iodide was used as a fluorescent probe for total cellular DNA and a monoclonal antibody against BrdUrd was used as a probe for BrdUrd incorporated into DNA. Fluorescein-labeled goat anti-mouse antibody was used to fluorescently label the bound anti-BrdUrd probe. Bivariate DNA/BrdUrd distributions measured for Chinese hamster ovary… CONTINUE READING
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