Flow cytometric kinetic assay of calcium mobilization in whole blood platelets using Fluo-3 and CD41.

@article{Monteiro1999FlowCK,
  title={Flow cytometric kinetic assay of calcium mobilization in whole blood platelets using Fluo-3 and CD41.},
  author={M do C{\'e}u Monteiro and Filipe Sansonetty and Maria Jos{\'e} Gonçalves and Jim E. O'Connor},
  journal={Cytometry},
  year={1999},
  volume={35 4},
  pages={302-10}
}
BACKGROUND Platelet activation plays a major role in the physiology and pathology of hemostasis. Flow cytometry is a promising approach for the structural and functional analysis of platelets. However, the choice of adequate biological parameters and most technical issues are still under discussion. A rise in cytosolic free Ca2+ is a key early event that follows platelet stimulation and precedes several activation responses, including shape change, aggregation, secretion, and expression of… CONTINUE READING

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