Flow cytometric evaluation of human basophils.

Abstract

The expression of CD45 and IgE cell surface antigens on human leucocytes was studied by flow cytometry. More than 80% of sorted cells that expressed low CD45 (CD45dim) and high IgE (IgEbright) antigen site density were identified as basophils. Immunomagnetic depletion of the CD45dim-IgEbright cell subset by a biotin-coupled anti-IgE antibody and streptavidin-coated magnetic beads was greater than 90%, and more than 80% of cells binding significant numbers of beads exhibited the morphological characteristics of basophils. Interestingly, when the cell staining was performed in the presence of calcium and magnesium, we observed a significant increase of CD45 and an equivalent decrease of IgE cell surface expression, as well as an IgE concentration dependent diminution of the number of CD45dim-IgEbright cells.

Statistics

0102030'97'99'01'03'05'07'09'11'13'15'17
Citations per Year

52 Citations

Semantic Scholar estimates that this publication has 52 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Gane1993FlowCE, title={Flow cytometric evaluation of human basophils.}, author={Pierre Gane and Catherine Pecquet and Patrick Lambin and Nisen Abuaf and Francisque Leynadier and Philippe Rouger}, journal={Cytometry}, year={1993}, volume={14 3}, pages={344-8} }