Flow cytometric estimation of the effect of Ginkgo biloba extract on the content of hydrogen peroxide in dissociated mammalian brain neurons.

@article{Oyama1992FlowCE,
  title={Flow cytometric estimation of the effect of Ginkgo biloba extract on the content of hydrogen peroxide in dissociated mammalian brain neurons.},
  author={Y. Oyama and T. Ueha and A. Hayashi and L. Chikahisa and K. Noda},
  journal={Japanese journal of pharmacology},
  year={1992},
  volume={60 4},
  pages={
          385-8
        }
}
The effect of Ginkgo biloba extract (GBE) on the content of hydrogen peroxide was estimated in cerebellar neurons dissociated from rats, by means of a flow-cytometer and 2',7'-dichlorofluorescein (DCF) diacetate, a fluorescent dye for intracellular hydrogen peroxide. The GBE started to reduce the DCF fluorescence of the neuron at 0.1 microgram/ml to 0.3 microgram/ml. Further increases in the GBE concentration (up to 3 micrograms/ml) produced a dose-dependent decrease in the DCF fluorescence… Expand
Ginkgo biloba extract protects brain neurons against oxidative stress induced by hydrogen peroxide
TLDR
Results implicate G. biloba extract as a potential agent in protecting the neurons suffering from oxidative stress induced by hydrogen peroxide. Expand
Preventive effect of Ginkgo biloba extract on apoptosis in rat cerebellar neuronal cells induced by hydroxyl radicals
TLDR
The results indicate that hydroxyl radicals induce apoptosis in rat cerebellar neuronal cells and this induction can be prevented by EGb. Expand
Ca(2+)-induced increase in oxidative metabolism of dissociated mammalian brain neurons: effect of extract of ginkgo biloba leaves.
TLDR
Effect of an extract of Ginkgo biloba leaves (EGb) on oxidative metabolism was studied using rat brain neurons and 2',7'-dichlorofluorescin fluorescence to suggest that EGb may reduce the Ca(2+)-induced increase in the oxidative metabolism of brain neurons. Expand
Myricetin and quercetin, the flavonoid constituents ofGinkgo biloba extract, greatly reduce oxidative metabolism in both resting and Ca2+-loaded brain neurons
TLDR
Incubation with myricetin or quercetin reduced the oxidation of DCFH in resting brain neurons, more profoundly than EGb, and may be responsible for a part of the beneficial effects of EGb on brain neurons subject to ischemia. Expand
Characterization of 2′,7′-dichlorofluorescin fluorescence in dissociated mammalian brain neurons: estimation on intracellular content of hydrogen peroxide
TLDR
The possibility that the DCF fluorescence may be useful in estimating the intracellular content of hydrogen peroxide of mammalian brain neurons is suggested. Expand
Extract of Ginkgo biloba leaves attenuates kainate-induced increase in intracellular Ca2+ concentration of rat cerebellar granule neurons.
TLDR
It is shown that EGBL exerts an inhibitory action on kainate receptor (a subtype of glutamate receptor) and may be very suitable for preventing and/or treating neurodegenerative diseases. Expand
Prevention of neuronal cell damage induced by oxidative stress in‐vitro: effect of different Ginkgo biloba extracts
TLDR
Observations make one suppose that different preparations of Ginkgo biloba have quantitatively different actions and outline the importance of the contribution of apoptosis prevention toward their neuroprotective action. Expand
Chemistry and Biological Activities of Ginkgo Biloba
ABSTRACT: Ginkgo biloba L. is the solo member of the Ginkgoaceae family, and its leaf extract (EGb) has been clinically used for the treatment of cerebral insufficiency. Several experimental studiesExpand
Structure-activity relationships of quercetin in antagonizing hydrogen peroxide-induced calcium dysregulation in PC12 cells.
TLDR
Data indicated that the particular polyphenolic structural components of quercetin provided its strong antioxidant property of protecting cells against H2O2-induced oxidative stress and calcium dysregulation. Expand
Effect of N,N-diethyldithiocarbamate on ionomycin-induced increase in oxidation of cellular 2′,7′-dichlorofluorescin in dissociated cerebellar neurons
TLDR
Results indicate that ionomycin increases the formation of superoxide anion in brain neuron, and DDC reduces the Ionomycin-induced augmentation of 2',7'-dichlorofluorescin fluorescence in a dose-dependent manner. Expand
...
1
2
3
4
...

References

SHOWING 1-10 OF 16 REFERENCES
Radical scavenging action of flunarizine in rat brain in vitro.
TLDR
The most pronounced activity of this compound was elicited in the mitochondria, where it was three times greater than that of alpha-tocopherol, a typical intracellular antioxidant, which suggests that the free radical scavenging or antioxidant activity of flunarizine may play a role in its brain protective action. Expand
Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation.
TLDR
The data indicate that the DCFH oxidation assay is quantitatively related to the oxidative metabolic burst of PMNL, and they strongly suggest that the reaction is mediated by H2O2 generated by the PMNL. Expand
Subpopulations of neutrophils with increased oxidative product formation in blood of patients with infection.
TLDR
The marked increase in oxidative metabolic capability of the primed PMNL may be a significant component of the host response to acute infection and contribute to the damage to host tissues such as pulmonary vascular endothelium during bacteremia. Expand
Free radicals in cerebral ischemia.
TLDR
The possibility that cerebral ischemia may initiate a series of pathological free radical reactions within the membrane components of the CNS was investigated in the cat and there was a progressive decrease in the amount of detectable ascorbic acid following middle cerebral artery occlusion. Expand
Independent stimulation of membrane potential changes and the oxidative metabolic burst in polymorphonuclear leukocytes.
TLDR
According to this data, membrane depolarization may antecede oxidative responses in PMNL, but appears to depend upon the method of cell preparation and the nature of the stimulus. Expand
Free radical damage of the brain following ischemia.
TLDR
The chapter demonstrates experiments; the rat brain surface emitted weak light promptly after embolization that lasted at least four hours, indicating the generation of excited species from an early stage of reoxygenation around multiple ischemic areas. Expand
Triphenyltin-induced increase in the intracellular Ca2+ of dissociated mammalian CNS neuron: its independence from voltage-dependent Ca2+ channels.
TLDR
TPT may promote Ca(2+)-influx to the neuron and affect the intracellular Ca-store sites, which are relevant to the neurotoxicity of organotins. Expand
Oxygen-derived free radicals in postischemic tissue injury.
  • J. McCord
  • Medicine
  • The New England journal of medicine
  • 1985
TLDR
It is now clear that oxygen-derived free radicals play an important part in several models of experimentally induced reperfusion injury, and Dysfunction induced by free radicals may be a major component of ischemic diseases of the heart, bowel, liver, kidney, and brain. Expand
Analysis and isolation of embryonic mammalian neurons by fluorescence- activated cell sorting
TLDR
The results demonstrate that a fluorescence-activated cell sorter can be used for quantitative analysis of specific cellular properties, that FACS analysis and sorting can beused to identify and isolate live cells from many regions of the embryonic mammalian CNS during important developmental periods, and that sorted neurons and glial cells can be maintained for weeks in culture. Expand
THE FLUOROMETRIC ANALYSIS OF ULTRAMICRO QUANTITIES OF HYDROGEN PEROXIDE.
TLDR
A fluorometric method is described for the analysis of hydrogen peroxide in the range of 10 −11 mole/ml based on the oxidation of nonfluorescent diacetyldichlorofluorescin to a fluorescent compound by hydrogenperoxide and peroxidase. Expand
...
1
2
...