Flow cytometric determination of genome size in Pinus

@article{OBrien1996FlowCD,
  title={Flow cytometric determination of genome size in Pinus},
  author={I. E. O'Brien and D. R. Smith and R. C. Gardner and B. Murray},
  journal={Plant Science},
  year={1996},
  volume={115},
  pages={91-99}
}
  • I. E. O'Brien, D. R. Smith, +1 author B. Murray
  • Published 1996
  • Biology
  • Plant Science
  • Abstract The nuclear 2C DNA content of seven Pinus species and Pseudotsuga menziesii was determined by flow cytometric analysis. Conditions for nuclear isolation were adapted to optimise results for Pinus . To reduce endonuclease activity and the effect of high tannins and phenolics, 10 mM EDTA, 15 mM β-mercaptoethanol and 1% PVP-10 were added to the chopping buffer. The accessibility of propidium iodide, an intercalating stain, was improved by acid treatment of the nuclei with HCl for 1 min… CONTINUE READING
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