• DOI:10.1016/0168-9452(96)04356-7
Corpus ID: 84745771

Flow cytometric determination of genome size in Pinus

@article{OBrien1996FlowCD,
  title={Flow cytometric determination of genome size in Pinus},
  author={I. E. O'Brien and D. R. Smith and R. C. Gardner and B. Murray},
  journal={Plant Science},
  year={1996},
  volume={115},
  pages={91-99}
}

I. E. O'Brien, D. R. Smith, +1 author B. Murray

Published 1996

Biology

Plant Science

Abstract The nuclear 2C DNA content of seven Pinus species and Pseudotsuga menziesii was determined by flow cytometric analysis. Conditions for nuclear isolation were adapted to optimise results for Pinus . To reduce endonuclease activity and the effect of high tannins and phenolics, 10 mM EDTA, 15 mM β-mercaptoethanol and 1% PVP-10 were added to the chopping buffer. The accessibility of propidium iodide, an intercalating stain, was improved by acid treatment of the nuclei with HCl for 1 min… CONTINUE READING

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