Flow cytometric determination of apoptosis in heterogeneous cell populations.

@article{Dolzhanskiy1995FlowCD,
  title={Flow cytometric determination of apoptosis in heterogeneous cell populations.},
  author={Alexandr Dolzhanskiy and Ross S. Basch},
  journal={Journal of immunological methods},
  year={1995},
  volume={180 1},
  pages={131-40}
}
We have shown that apoptosis and surface antigen expression can be detected simultaneously using multicolor flow cytometry. Apoptosis was measured with an in situ assay that makes use of the ability of the enzyme terminal deoxyribonucleotidyl transferase to catalyze the addition of biotinylated nucleotides to the free 3'-OH groups produced during… CONTINUE READING