Flow cytometric detection of tandem repeat mutations induced by various chemical classes.

@article{Healy2006FlowCD,
  title={Flow cytometric detection of tandem repeat mutations induced by various chemical classes.},
  author={Caroline Healy and Mike J Wade and Avril McMahon and Andrew L. Williams and Douglas A. Johnson and Craig L J Parfett},
  journal={Mutation research},
  year={2006},
  volume={598 1-2},
  pages={85-102}
}
To facilitate detection of genotoxicity from environmental mutagen exposure, we generated an in vitro enhanced green fluorescence protein (EGFP) reactivation assay that quickly and effectively detects frameshift mutations in tandem repeat sequences (TRS). Two murine cell lines, C3H10T1/2 and mismatch repair deficient MC2a, were stably transfected with EGFP reporter plasmids in which the EGFP constructs contain TRS that put the EGFP sequence out of frame. These included several 2, 3, 4, 5 and 6… CONTINUE READING