Flow cytometric detection of mitochondrial dysfunction in subpopulations of human mononuclear cells.

@article{Kunz1997FlowCD,
  title={Flow cytometric detection of mitochondrial dysfunction in subpopulations of human mononuclear cells.},
  author={Dagmar Kunz and Claus W Luley and Kirstin Winkler and Hartmut Lins and Wolfram S Kunz},
  journal={Analytical biochemistry},
  year={1997},
  volume={246 2},
  pages={218-24}
}
At 488 nm argon-ion laser excitation human mononuclear cells emit flavoprotein-related autofluorescence signals. Approximately 60% of these are caused by the mitochondrial flavoproteins alpha-lipoamide dehydrogenase and electron transfer flavoprotein, having differences in their fluorescence emission spectra. At the emission wavelength of 530 nm the redox changes of alpha-lipoamide dehydrogenase fluorescence in human mononuclear cells can be monitored by flow cytometry. This allows the… CONTINUE READING
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