Flow cytometric cell division tracking using nuclei.

  title={Flow cytometric cell division tracking using nuclei.},
  author={Jhagvaral Hasbold and Philip D. Hodgkin},
  volume={40 3},
BACKGROUND Labeling cells with 5-(and-6) carboxyfluorescein diacetate succinimidyl ester (CFSE) allows their subsequent division history to be determined by flow cytometry. Whether nuclei isolated from CFSE-labeled cells retain any or sufficient dye to reveal the same division history was unknown. If division tracking in nuclei were possible, it would… CONTINUE READING