Flow cytometric calcium flux assay: evaluation of cytoplasmic calcium kinetics in whole blood leukocytes.


In leukocytes, as in many other cell types, cytoplasmic calcium ([Ca(2+)](i)) changes play a key role in a series of pathways leading to activation. Here we describe a flow cytometric method allowing the simultaneous kinetic analysis of changes in [Ca(2+)](i) in the three types of leukocytes, i.e. monocytes, granulocytes and lymphocytes. Heparinised whole… (More)
DOI: 10.1016/j.jim.2009.07.002


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