Flow cytometric and microscopic analysis of the effect of tannic acid on plant nuclei and estimation of DNA content.

@article{Loureiro2006FlowCA,
  title={Flow cytometric and microscopic analysis of the effect of tannic acid on plant nuclei and estimation of DNA content.},
  author={Jo{\~a}o Loureiro and Eleazar Rodriguez and Jaroslav Dole{\vz}el and Conceiç{\~a}o Santos},
  journal={Annals of botany},
  year={2006},
  volume={98 3},
  pages={515-27}
}
BACKGROUND AND AIMS Flow cytometry (FCM) is extensively used to estimate DNA ploidy and genome size in plants. In order to determine nuclear DNA content, nuclei in suspension are stained by a DNA-specific fluorochrome and fluorescence emission is quantified. Recent studies have shown that cytosolic compounds may interfere with binding of fluorochromes to DNA, leading to flawed data. Tannic acid, a common phenolic compound, may be responsible for some of the stoichiometric errors, especially in… CONTINUE READING
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