Flow cytometric analysis of within-strain variation in polysaccharide expression by Bacteroides fragilis by use of murine monoclonal antibodies.

@article{Lutton1991FlowCA,
  title={Flow cytometric analysis of within-strain variation in polysaccharide expression by Bacteroides fragilis by use of murine monoclonal antibodies.},
  author={Dale Lutton and Sheila Patrick and Alistair D. Crockard and Lorna M. D Stewart and Michael J. Larkin and Evelyn Dermott and Thomas A McNeill},
  journal={Journal of medical microbiology},
  year={1991},
  volume={35 4},
  pages={229-37}
}
The reactivity of four different monoclonal antibodies (MAbs) with populations of Bacteroides fragilis NCTC 9343, enriched by density gradient centrifugation for a large capsule, small capsule and electron-dense layer (EDL) only visible by electronmicroscopy, was examined. The MAbs reacted strongly with polysaccharides present in both the large capsule- and EDL-enriched populations but not in the small capsule-enriched populations. The pattern of labelling was determined by immunoblotting… CONTINUE READING
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