Flow cytometric analysis of transmembrane phospholipid movement in bull sperm.

@article{Nolan1995FlowCA,
  title={Flow cytometric analysis of transmembrane phospholipid movement in bull sperm.},
  author={John P Nolan and S F Magargee and Richard G. Posner and Roy H. Hammerstedt},
  journal={Biochemistry},
  year={1995},
  volume={34 12},
  pages={
          3907-15
        }
}
Fluorescent phospholipids are useful to investigate phospholipid dynamics in biological membranes. We used flow cytometry to investigate transbilayer phospholipid movement in live sperm cells. Acyl-labeled N-4-nitrobenzo-2-oxa-1,3-diazole (NBD) -phosphatidylcholine (-PC), -phosphatidylethanolamine (-PE), or -phosphatidylserine (-PS) were incorporated into sperm cells, and the transbilayer location was determined by extraction of probe from cell with excess bovine serum albumin (BSA) or by… 
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