Flow cytometric analysis of porcine preadipocyte replication.

  title={Flow cytometric analysis of porcine preadipocyte replication.},
  author={J. Thomas Wright and G. J. Hausman},
  journal={Journal of animal science},
  volume={72 7},
In the present study, a technique is described for measuring proliferation of primary porcine preadipocytes. Cultures of stromal-vascular (S-V) cells from dorsal subcutaneous adipose tissue were maintained under several growth conditions. Cells were isolated from cultures at various times and DNA was labeled with propidium iodide (PI) and analyzed by flow cytometry. Treatment of cultures with colchicine allowed identification of S-V cells in mitosis. The relative proportion of replicating cells… CONTINUE READING

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