Flow cytometric analysis of neutrophils in cows' milk.

  title={Flow cytometric analysis of neutrophils in cows' milk.},
  author={Ronald H. Miller and Max J. Paape and R Filep and Susanne Link},
  journal={American journal of veterinary research},
  volume={54 12},
Procedures were developed to count neutrophils in milk, using a flow cytometer. Milk samples from 2 experiments were counted: 1 with 4 noninfected cows and a second with 5 noninfected cows that were injected with endotoxin in 2 mammary quarters. Thus, the procedures were evaluated on normal milk and on that with high somatic cell count. Flow cytometric procedures involved fluorescence detection (from the dye carboxydimethylfluorescein diacetate) to distinguish intact and viable from fragmented… CONTINUE READING