Flow cytometric analysis of in vitro bluetongue virus infection of bovine blood mononuclear cells.

@article{BarrattBoyes1992FlowCA,
  title={Flow cytometric analysis of in vitro bluetongue virus infection of bovine blood mononuclear cells.},
  author={Simon M Barratt-Boyes and Paul V. Rossitto and Jeffrey L. Stott and N. James Maclachlan},
  journal={The Journal of general virology},
  year={1992},
  volume={73 ( Pt 8)},
  pages={1953-60}
}
Cultures of adherent and non-adherent bovine peripheral blood mononuclear (PBM) cells were inoculated with bluetongue virus (BTV) serotype 10. Some cultures of non-adherent cells were stimulated with interleukin 2 (IL-2) and concanavalin A for 24 h prior to virus inoculation. Cells were harvested at various intervals up to 72 h after inoculation. A panel of leukocyte differentiation antigen-specific monoclonal antibodies (MAbs), specific for bovine CD2, CD4 or CD8, monocytes and granulocytes, B… CONTINUE READING
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