Flow cytometric analysis of cell division history using dilution of carboxyfluorescein diacetate succinimidyl ester, a stably integrated fluorescent probe.

@article{Lyons2001FlowCA,
  title={Flow cytometric analysis of cell division history using dilution of carboxyfluorescein diacetate succinimidyl ester, a stably integrated fluorescent probe.},
  author={Alan Bruce Lyons and Jhagvaral Hasbold and Philip D. Hodgkin},
  journal={Methods in cell biology},
  year={2001},
  volume={63},
  pages={
          375-98
        }
}
Publisher Summary This chapter reviews the use of a technique based on the serial dilution of a stably binding intracellular fluorochrome, carboxyfluorescein diacetate succinimidyl ester (CFSE), which allows eight to ten sequential cell divisions to be analyzed by flow cytometry. When incubated with cells, the fluorescein-based CFSE crosses the cell membrane and attaches to free amine groups of cytoplasmic cell proteins. After enzymatic removal of carboxyl groups by endogenous intracellular… CONTINUE READING
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