Flow-cytometric analysis of basophil activation: inhibition by histamine at conventional and homeopathic concentrations

  title={Flow-cytometric analysis of basophil activation: inhibition by histamine at conventional and homeopathic concentrations},
  author={Vanessa Brown and Madeleine Ennis},
  journal={Inflammation Research},
Human basophils play a pivotal role in the pathogenesis of chronic allergic diseases such as asthma. Activation of basophils by cross-linking of IgE induces fusion of the cytoplasmic granules with the plasma membrane and the subsequent release of potent mediators including histamine. Basophil activation can be quantified by the measurement of secreted histamine [1] and by direct microscopic examination of the percentage of degranulated cells [2]. More recently, flow cytometry has been used to… 

Sensitive Flow Cytometric Method to Test Basophil Activation Influenced by Homeopathic Histamine Dilutions

A 3-color flow cytometric method with enhanced accuracy was established to measure the effects from homeopathic solutions in an in-vitro cell system and documented an unequivocally inhibited basophil activation by anti-IgE.

Inhibition of CD203c membrane up-regulation in human basophils by high dilutions of histamine: a controlled replication study

It is shown that low and high dilutions of histamine inhibit CD203c up-regulation in anti-IgE stimulated basophils, using a strictly standardized flow cytometry protocol and a new dilution/succussion procedure.

Original Research Papers Histamine dilutions modulate basophil activation

High dilutions of histamine may indeed exert an effect on basophil activity and this activity observed by staining basophils with alcian blue was confirmed by flow cytometry.

Histamine dilutions modulate basophil activation

In 3 different types of experiment, it has been shown that high dilutions of histamine may indeed exert an effect on basophil activity, with the greatest inhibitory effects seen at lower levels of stimulation.

Basophil models of homeopathy: a sceptical view.

  • M. Ennis
  • Biology
    Homeopathy : the journal of the Faculty of Homeopathy
  • 2010

Differential effect of storage on molecular and ultra-molecular dilutions of histamine

The effect of storage on the activity of these histamine dilutions is presented, which led to the confirmation that high dilutions of histamine were capable of inhibiting anti-IgE or fMLP induced basophil activation.

Influence of the diluent on the effect of highly diluted histamine on basophil activation.

The preliminary results support the hypothesis that the dilution medium may influence the effects of high dilutions, of importance for homeopathic pharmaceutical practice as well as for ultra-high dilution experiments.

Survey of immuno-allergological ultra high dilution research.

  • B. Poitevin
  • Biology, Medicine
    Homeopathy : the journal of the Faculty of Homeopathy
  • 2015



Monitoring human basophil activation via CD63 monoclonal antibody 435.

Limiting concentrations of human basophil-bound IgE antibody required for histamine release.

Since percentage histamine release and percentage degranulated cells were highly correlated after suboptimal sensitization, individual cells or subsets of basophils were apparently differentially responsive, consistent with other evidence of functional heterogeneity and that Histamine release by individual cells is an all or none process.

Basophil counting with a new staining method using alcian blue.

The improved reproducibility, accuracy, and convenience of this method over existing methods should facilitate the collection of more meaningful information about circulating basophil levels in health and disease.

Analyse de l’expression membranaire du marquer CD63 par activation du basophile humain. Aplication au diagnostic allergologique

  • Allergie et Immunologie
  • 1994