Flow-cytometric analysis of basophil activation: inhibition by histamine at conventional and homeopathic concentrations

@article{Brown2001FlowcytometricAO,
  title={Flow-cytometric analysis of basophil activation: inhibition by histamine at conventional and homeopathic concentrations},
  author={Vanessa Brown and Madeleine Ennis},
  journal={Inflammation Research},
  year={2001},
  volume={50},
  pages={47-48}
}
Human basophils play a pivotal role in the pathogenesis of chronic allergic diseases such as asthma. Activation of basophils by cross-linking of IgE induces fusion of the cytoplasmic granules with the plasma membrane and the subsequent release of potent mediators including histamine. Basophil activation can be quantified by the measurement of secreted histamine [1] and by direct microscopic examination of the percentage of degranulated cells [2]. More recently, flow cytometry has been used to… 

Sensitive Flow Cytometric Method to Test Basophil Activation Influenced by Homeopathic Histamine Dilutions

A 3-color flow cytometric method with enhanced accuracy was established to measure the effects from homeopathic solutions in an in-vitro cell system and documented an unequivocally inhibited basophil activation by anti-IgE.

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High dilutions of histamine may indeed exert an effect on basophil activity and this activity observed by staining basophils with alcian blue was confirmed by flow cytometry.

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In 3 different types of experiment, it has been shown that high dilutions of histamine may indeed exert an effect on basophil activity, with the greatest inhibitory effects seen at lower levels of stimulation.

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The preliminary results support the hypothesis that the dilution medium may influence the effects of high dilutions, of importance for homeopathic pharmaceutical practice as well as for ultra-high dilution experiments.

Survey of immuno-allergological ultra high dilution research.

  • B. Poitevin
  • Biology, Medicine
    Homeopathy : the journal of the Faculty of Homeopathy
  • 2015
...

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