Flow cytometric allergy diagnosis: basophil activation techniques.


The basis of flow cytometric allergy diagnosis is quantification of changes in expression of basophilic surface membrane markers (Ebo et al., Clin Exp Allergy 34: 332-339, 2004). Upon encountering specific allergens recognized by surface receptor FcεRI-bound IgE, basophils not only secrete and generate quantifiable bioactive mediators but also up-regulate the expression of different markers (e.g., CD63, CD203c) which can be detected by multicolor flow cytometry using specific monoclonal antibodies (Ebo et al., Cytometry B Clin Cytom 74: 201-210, 2008). Here, we describe two flow cytometry-based protocols which allow detection of surface marker activation (Method 1) and changes in intragranular histamine (Method 2), both reflecting different facets of basophil activation.

DOI: 10.1007/978-1-4939-1173-8_11

Cite this paper

@article{Bridts2014FlowCA, title={Flow cytometric allergy diagnosis: basophil activation techniques.}, author={Chris H. Bridts and Vito Sabato and Christel H Mertens and Margo M. Hagendorens and Luc S. De Clerck and Didier G Ebo}, journal={Methods in molecular biology}, year={2014}, volume={1192}, pages={147-59} }