Flow cytometric CD34+ stem cell enumeration: lessons from nine years' external quality assessment within the Benelux countries.

@article{Levering2007FlowCC,
  title={Flow cytometric CD34+ stem cell enumeration: lessons from nine years' external quality assessment within the Benelux countries.},
  author={Wilfried H B M Levering and Frank W. M. B. Preijers and Wessel N van Wieringen and J. K. Kraan and Wil A M van Beers and Kees Sintnicolaas and Dick J van Rhenen and Jan Willem C. Gratama},
  journal={Cytometry. Part B, Clinical cytometry},
  year={2007},
  volume={72 3},
  pages={178-88}
}
BACKGROUND A biannual external quality assurance (EQA) scheme for flow cytometric CD34+ haematopoietic stem cell enumeration has been operational in the Benelux countries since 1996. In an evaluation of the results of 16 send-outs, we studied the effects of the methods used on assay outcome and whether or not this exercise was effective in reducing between-laboratory variation. METHODS Data were analyzed using robust multivariate regression. This approach is relatively insensitive to outliers… CONTINUE READING

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