Flow acetone-staining technique: a highly efficient procedure for the simultaneous analysis of DNA content, cell morphology, and immunophenotype by flow cytometry.

@article{Carbonari2008FlowAT,
  title={Flow acetone-staining technique: a highly efficient procedure for the simultaneous analysis of DNA content, cell morphology, and immunophenotype by flow cytometry.},
  author={Maurizio Carbonari and Debora Mancaniello and Tiziana Tedesco and Massimo Fiorilli},
  journal={Cytometry. Part A : the journal of the International Society for Analytical Cytology},
  year={2008},
  volume={73 2},
  pages={168-74}
}
The accurate determination of cell cycle, immunophenotypes and morphology at single-cell level is not fully achieved by current flow cytometry protocols. Acetone, a coagulant fixative/permealizing agent, is widely used in static cytometry, but is impractical in flow cytometry because of its shrinking effect. We sought for conditions of acetone treatment that could permit the simultaneous analysis of physical parameters, surface and intracellular immunostaining, and DNA content. We evaluated… CONTINUE READING