Flow Cytometry Reveals Similarities Between Lung Macrophages in Humans and Mice.

@article{Bharat2016FlowCR,
  title={Flow Cytometry Reveals Similarities Between Lung Macrophages in Humans and Mice.},
  author={Ankit Bharat and Sangeeta M. Bhorade and Luisa I Morales-Nebreda and Alexandra C McQuattie-Pimentel and Saul Soberanes and Karen Ridge and Malcolm M Decamp and Karen K L Mestan and Harris Perlman and G. R. Scott Budinger and Alexander V Misharin},
  journal={American journal of respiratory cell and molecular biology},
  year={2016},
  volume={54 1},
  pages={147-9}
}
Findings in murine models implicate subpopulations of alveolar macrophages in the pathogenesis of lung injury and fibrosis, however, the relevance of these findings for humans with chronic lung disease is unknown in part due to a lack of proper tools to identify macrophage heterogeneity in the human lung. Here we report a flow cytometry protocol that allows unambiguous identification of alveolar macrophages, interstitial macrophages and monocytes in the human lung and in bronchoalveolar lavage… CONTINUE READING