Flow CytometricMeasurementof Peptidaseswith Use of 5-Nitrosalicylaldehydeand 4-Methoxy-f 3-NaphthylamineDerivatives

Abstract

Enzyme activity can be measured in single cells or in cell suspensions by either static or flow microfluorometry when a fluorogenic substrate is used. We have used amino acid derivatives of arylamines as fluorogenic substrates for tagging cellular proteinases. The liberated aromatic amine, which can diffuse from the cell, is trapped as a fluorescent… (More)

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