Flow Cytometric Characteristics of Sperm Cells Isolated from Pollen of Zea mays L.

@article{Zhang1992FlowCC,
  title={Flow Cytometric Characteristics of Sperm Cells Isolated from Pollen of Zea mays L.},
  author={G. S. Zhang and M K Campenot and L. E. McGann and David D. Cass},
  journal={Plant physiology},
  year={1992},
  volume={99 1},
  pages={
          54-9
        }
}
Sperm cells have been isolated from pollen of maize (Zea mays L.) and purified with Percoll density centrifugation. Their flow cytometric characteristics were determined on a FACScan flow cytometer with the fluorescent dyes, fluorescein diacetate and propidium iodide. Freshly isolated sperm cells appeared as a dot cluster on the forward scatter and side scatter dot plot. This dot cluster contained 85 to 95% of the 10 thousand counts collected. More than 98% of cells from the cluster were… 
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Methods Citations
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33 Citations

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Examination of morphological changes of isolated maize sperm cells in the presence of Brewbaker and Kwack salts or the individual components of BKS revealed two or more nuclei in a single cell, suggesting that BKS induces cell fusion.
Detection of changes in the nuclear phase and evaluation of male germ units by flow cytometry during in vitro pollen tube growth in Alstroemeria aurea
TLDR
Analysis of male gamete behavior from mature pollen to pollen tube growth in the bicellular pollen species Alstroemeria aurea revealed changes in the nuclear phase at 9 and 18 h after culture, indicating that the first change was due to the division of the generative cells into sperm cells.
RNA and protein synthesis in sperm cells isolated from Zea mays L. pollen
TLDR
It is possible that the differences in synthetic activity between freshly isolated and 24-h-old cells might correspond to sperm cell activation during pollen tube growth, and if so, these metabolic changes may play an important role in fertilization.
Effects of calcium, magnesium, potassium and boron on sperm cells isolated from pollen of Zea mays L.
TLDR
The results collectively suggest that maize sperm cells are sensitive to exogenous free calcium; however, a certain level of cytosolic free calcium is necessary for maintenance of integrity.
Flow cytometric analysis of pollen and spores: An overview of applications and methodology
TLDR
The discussion here mostly concerns pollen, but the general principles described here can be extended to apply to spores in ferns, lycophytes, and bryophytes.
Direct analysis of pollen fitness by flow cytometry: implications for pollen response to stress.
TLDR
The ability to use flow cytometry to quantify ROS dynamics within a large pollen population was shown by dose-dependent alterations in DCF-fluorescence in response to oxidative stress or antioxidant treatments, which correlated with a ~60% reduction in pollen germination.
Proteome and Transcriptional Analysis of Arabidopsis thaliana Sperm Cells
TLDR
For the first time, sperm cell size dimorphism was identified for Arabidopsis in this study utilising a GFP-labelled sperm line and confocal microscopy and will certainly facilitate further sperm cell-specific gene identification studies.
Identification of Proteins Enriched in Rice Egg or Sperm Cells by Single-Cell Proteomics
TLDR
The results suggested that the proteomic data presented in this study are foundational information toward understanding the mechanisms of reproduction and early development in angiosperms.
Microscopy of Physcomitrella patens sperm cells
BackgroundArchegoniates (bryophytes, ferns and gymnosperms), such as the moss Physcomitrella patens, possess freely motile sperm cells (spermatozoids) which reach the egg cell via surface water.…
Characterization of calcium uptake in isolated maize sperm cells
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References

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TLDR
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TLDR
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TLDR
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