Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10.

@article{Sring2016FlowCA,
  title={Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10.},
  author={Katrin S{\"u}ring and Sabine Bach and Conny Hoeflich and Wolfgang Straff},
  journal={Journal of visualized experiments : JoVE},
  year={2016},
  volume={117}
}
Flow cytometry is a method widely used to quantify suspended solids such as cells or bacteria in a size range from 0.5 to several tens of micrometers in diameter. In addition to a characterization of forward and sideward scatter properties, it enables the use of fluorescent labeled markers like antibodies to detect respective structures. Using indirect antibody staining, flow cytometry is employed here to quantify birch pollen allergen (precisely Bet v 1)-loaded particles of 0.5 to 10 µm in… CONTINUE READING