Flow Cytometric Analysis of Characteristics of Hybridization of Species-Specific Fluorescent Oligonucleotide Probes to rRNA of Marine Nanoflagellates.

@article{Rice1997FlowCA,
  title={Flow Cytometric Analysis of Characteristics of Hybridization of Species-Specific Fluorescent Oligonucleotide Probes to rRNA of Marine Nanoflagellates.},
  author={Joe Rice and Michael A. Sleigh and Peter H. Burkill and Glen A. Tarran and C. David O'Connor and Mikhail V Zubkov},
  journal={Applied and environmental microbiology},
  year={1997},
  volume={63 3},
  pages={938-44}
}
Identification problems restrict quantitative ecological research on specific nanoflagellates. Identification by specific oligonucleotide probes permits use of flow cytometry for enumeration and measurement of size of nanoflagellates in statistically meaningful samples. Flow cytometry also permits measurement of intensity of probe binding by cells. Five fluorescent probes targeted to different regions of the small subunit rRNA of the common marine flagellate Paraphysomonas vestita all… CONTINUE READING

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