Flow Cytometric Analysis of Cellular Berberine Contents in High- and Low-Producing Cell Lines of Coptis japonica Obtained by Repeated Selection.

  title={Flow Cytometric Analysis of Cellular Berberine Contents in High- and Low-Producing Cell Lines of Coptis japonica Obtained by Repeated Selection.},
  author={Yasuhiro Hara and Hikaru Yamagata and Teijiro Morimoto and Junzou Hiratsuka and Toshihiro Yoshioka and Yasuhiro Fujita and Y. Yamada},
  journal={Planta medica},
  volume={55 2},
Cell lines that were highly productive for berberine were selected by repeated cloning of small cell aggregates. The berberine content of the highest-producing cell line increased after 4 clonings to 10% dw in comparison to the 3% dw found for the parent line, and the berberine yield was about 1,500 mg/1/14 days. There was no increase in berberine yield after the fifth cloning. Low-producing cell lines also appeared, even as the progeny of a highly productive cell line. We investigated the… Expand
Stimulation of berberine secretion and growth in cell cultures of Thalictrum minus
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Flow cytometry in plant breeding
  • S. Ochatt
  • Biology, Medicine
  • Cytometry. Part A : the journal of the International Society for Analytical Cytology
  • 2008
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