Flow‐Synthesis of Nucleosides Catalyzed by an Immobilized Purine Nucleoside Phosphorylase from Aeromonas hydrophila: Integrated Systems of Reaction Control and Product Purification

  title={Flow‐Synthesis of Nucleosides Catalyzed by an Immobilized Purine Nucleoside Phosphorylase from Aeromonas hydrophila: Integrated Systems of Reaction Control and Product Purification},
  author={Enrica Calleri and Giulia Cattaneo and Marco Rabuffetti and Immacolata Serra and Teodora Bavaro and Gabriella Massolini and Giovanna Speranza and Daniela Ubiali},
  journal={Advanced Synthesis \& Catalysis},
Analytical settings for in-flow biocatalytic reaction monitoring
This review explores the recent achievements on the development and applications of different analytical set-up for the in-line and on-line monitoring of in-flow bioreactions and illustrates that integration of IMERs in analytical platforms may widespread the industrial applications of biocatalyzed reactions.
Design of epidermal growth factor immobilization on 3D biocompatible scaffolds to promote tissue repair and regeneration
Bioconjugates based on hEGF mimicking the protein in its native state and thus suitable for tissue engineering applications, in particular for treating skin-related disorders as burns are developed.
Immobilized-Enzyme Reactors Integrated into Analytical Platforms: Recent Advances and Challenges
A critical discussion of challenges often encountered in IMER fabrication, sample preparation, integration into the analytical workflow, long-term usage, and of potential ways to overcome these is provided.
Continuous Flow Organophosphorus Chemistry
Immobilized enzyme reactors based on nucleoside phosphorylases and 2'-deoxyribosyltransferase for the in-flow synthesis of pharmaceutically relevant nucleoside analogues.
Both IMERs proved to be promising alternatives to chemical routes for the synthesis of nucleoside analogues and the developed in-flow system represents a powerful tool for the fast production on analytical scale ofucleosides for preliminary biological tests.
Novel Tricyclic 2-Alkoxy-8-methyl-6-(pyrrolidin-1-yl)-4H-[1,2,4]triazolo[5,1-f]purine Derivatives: Synthesis and Characterization
A convenient approach for the synthesis of diversely functionalized [1,2,4]triazolo[5,1-f]purine heterocyclic framework have been accomplished. The products were obtained through the combination of...
Prediction of kinetics and yields of nucleoside phosphorylase reactions and derived enzymatic reaction cascades
Reactions catalyzed by nucleoside phosphorylases are attractive routes towards the production of pharmaceutically interesting nucleoside compounds. Many different nucleoside phosphorylases were
Bio-catalytic synthesis of unnatural nucleosides possessing a large functional group such as a fluorescent molecule by purine nucleoside phosphorylase
Unnatural nucleosides are attracting interest as potential diagnostic tools, medicines, and functional molecules.
Chemoenzymatic synthesis of nucleoside analogs as potential medicinal agents
I Abstract Modified nucleosides are important drugs used to treat cancer, viral or bacterial infections. They also serve as precursors for the synthesis of modified oligonucleotides (antisense


Redesigning the synthesis of vidarabine via a multienzymatic reaction catalyzed by immobilized nucleoside phosphorylases
We here report on the enzymatic synthesis of the antiviral drug vidarabine (arabinosyladenine, araA) starting from arabinosyluracil and adenine. To this aim, uridine phosphorylase from Clostridium
Biocatalysis as a Strategic Green Technology for the Chemical Industry
SIBC is a consortium set up to share industrial enzyme and strain collections for the mutual benefit of members. Each member company has an enzyme and/or strain collection, of which more than 99 %
Immobilized purine nucleoside phosphorylase from Aeromonas hydrophila as an on-line enzyme reactor for biocatalytic applications.
  • E. Calleri, D. Ubiali, +5 authors G. Massolini
  • Chemistry, Medicine
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
  • 2014
Comparison with the soluble enzyme showed that the AhPNP-based bioreactor is reliable as the same ranking order, with respect to the standard activity assay, was obtained.
The chemoenzymatic synthesis of clofarabine and related 2′-deoxyfluoroarabinosyl nucleosides: the electronic and stereochemical factors determining substrate recognition by E. coli nucleoside phosphorylases
Two approaches to the synthesis of 2-chloro-9-(2-deoxy-2-fluoro-β-D-arabinofuranosyl)adenine (1, clofarabine) were studied and differences of the reactivity of various substrates were analyzed by ab initio calculations.
Biotransformation with whole microbial systems in a continuous flow reactor: resolution of (RS)-flurbiprofen using Aspergillus oryzae by direct esterification with ethanol in organic solvent
Abstract Cell-bound lipases of dry mycelium of Aspergillus oryzae were used in organic solvent for the resolution of racemic flurbiprofen by direct esterification with ethanol in a flow-chemistry
Developing a Collection of Immobilized Nucleoside Phosphorylases for the Preparation of Nucleoside Analogues: Enzymatic Synthesis of Arabinosyladenine and 2,3-Dideoxyinosine
The use of nucleoside phosphorylases (NPs; EC 2.4.2.n) represents a convenient alternative to the chemical route for the synthesis of natural and modified nucleosides. We purified four recombinantly
Microfluidic multi-input reactor for biocatalytic synthesis using transketolase☆
Graphical abstract
Recombinant purine nucleoside phosphorylases from thermophiles: preparation, properties and activity towards purine and pyrimidine nucleosides
The results reveal that the structure of the heterocyclic base (e.g. adenine or hypoxanthine) can play a critical role in the phosphorolysis reaction, and may be helpful for reaction mechanism studies or optimization of reaction conditions.
Comparative investigations on thermostable pyrimidine nucleoside phosphorylases from Geobacillus thermoglucosidasius and Thermus thermophilus
Abstract The recombinant expression and biocatalytic characterization of two thermostable pyrimidine nucleoside phosphorylases (PyNP), isolated from Geobacillus thermoglucosidasius (Gt) and Thermus