Flounder growth hormone: isolation, characterization, and effects on growth of juvenile rainbow trout.

@article{Sakata1993FlounderGH,
  title={Flounder growth hormone: isolation, characterization, and effects on growth of juvenile rainbow trout.},
  author={Shusaku Sakata and T Noso and Shunsuke Moriyama and Tetsuya Hirano and Hiroshi Kawauchi},
  journal={General and comparative endocrinology},
  year={1993},
  volume={89 3},
  pages={396-404}
}
A highly purified growth hormone (GH) was isolated from the pituitary of the Japanese flounder (Paralichthys olivaceus) following extraction with alkaline solution, gel filtration on Sephadex G-75, and high-performance liquid chromatography (HPLC) on a reverse-phase column. Flounder GH (flGH) has a molecular weight of 20 kDa determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and an isoelectric point of 7.1 by gel electrofocusing. The complete amino acid sequence… CONTINUE READING