Floral transformation of wheat.

@article{Agarwal2009FloralTO,
  title={Floral transformation of wheat.},
  author={Sujata Agarwal and Star Loar and Camille M Steber and Janice Zale},
  journal={Methods in molecular biology},
  year={2009},
  volume={478},
  pages={105-13}
}
A method is described for the floral transformation of wheat using a protocol similar to the floral dip of Arabidopsis. This method does not employ tissue culture of dissected embryos, but instead pre-anthesis spikes with clipped florets at the early, mid to late uninucleate microspore stage are dipped in Agrobacterium infiltration media harboring a vector carrying anthocyanin reporters and the NPTII selectable marker. T1 seeds are examined for color changes induced in the embryo by the… CONTINUE READING