Floral spray transformation can efficiently generate Arabidopsis transgenic plants.

@article{Chung2000FloralST,
  title={Floral spray transformation can efficiently generate Arabidopsis transgenic plants.},
  author={Mi Hee Chung and M. K. Chen and Shu Mei Pan},
  journal={Transgenic research},
  year={2000},
  volume={9 6},
  pages={471-6}
}
In this study, floral spray and floral dip were used to replace the vacuum step in the Agrobacterium-mediated transformation of a superoxide dismutase (SOD) gene into Arabidopsis. The transgene was constructed by using a CaMV 35S promoter to drive a rice cytosolic CuZnSOD coding sequence in Arabidopsis. The transgene construct was developed in binary vectors and mobilized into Agrobacterium. When Arabidopsis plants started to initiate flower buds, the primary inflorescence shoots were removed… CONTINUE READING