Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.

@article{Clough1998FloralDA,
  title={Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.},
  author={Steven J Clough and Andrew F. Bent},
  journal={The Plant journal : for cell and molecular biology},
  year={1998},
  volume={16 6},
  pages={
          735-43
        }
}
  • S. CloughA. Bent
  • Published 1 December 1998
  • Biology
  • The Plant journal : for cell and molecular biology
The Agrobacterium vacuum infiltration method has made it possible to transform Arabidopsis thaliana without plant tissue culture or regeneration. [] Key Result Sucrose and surfactant were critical to the success of the floral dip method. Plants inoculated when numerous immature floral buds and few siliques were present produced transformed progeny at the highest rate.

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Minor modifications in obtainable Arabidopsis floral dip method enhances transformation efficiency and production of homozygous transgenic lines harboring a single copy of transgene

Modified protocol produced ~12-14% increase in transformants and increased the percentage of homozygous and single copied stable transgenic lines in Arabidopsis thaliana.

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