Flocculation onset, growth phase, and genealogical age in Saccharomyces cerevisiae.

@article{Soares1996FlocculationOG,
  title={Flocculation onset, growth phase, and genealogical age in Saccharomyces cerevisiae.},
  author={Eduardo V. Soares and Manuel Mota},
  journal={Canadian journal of microbiology},
  year={1996},
  volume={42 6},
  pages={
          539-47
        }
}
  • E. V. Soares, M. Mota
  • Published 1 June 1996
  • Biology, Materials Science
  • Canadian journal of microbiology
Flocculation onset, the time during the fermentative cycle at which the strains of Saccharomyces cerevisiae become flocculent, is an important factor in the brewing industry. The flocculation ability of Flo 1 phenotype (strain NCYC 869) remained practically unchanged throughout the growth and seems to be insensitive to the presence of nutrients of the culture medium. On the contrary, the flocculation of NewFlo phenotype (strain NCYC 1195) exhibited a cyclic behaviour. It was found that the loss… 
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References

SHOWING 1-10 OF 64 REFERENCES
Effect of cultural and nutritional conditions on the control of flocculation expression in Saccharomyces cerevisiae.
TLDR
It was clearly demonstrated that the phenotypic expression of flocculation could be induced or repressed by changing cultural and nutritional conditions.
Flocculence of Saccharomyces cerevisiae cells is induced by nutrient limitation, with cell surface hydrophobicity as a major determinant
TLDR
Initiation of flocculation ability of Saccharomyces cerevisiae MPY1 cells was observed at the moment the cells stop dividing because of nitrogen limitation, and a proteinaceous cell surface factor(s) was identified as a flocculin which appears to be involved in both cell surface hydrophobicity and in flocculating ability of the yeast cells.
Genetic Control of Flocculation
TLDR
The term “nonflocculent” will, therefore, imply the lack of cell aggregation and consequently much slower separation of (dispersed) yeast cells from the liquid medium.
THE EFFECT OF NITROGENOUS SUBSTANCES ON THE TIME OF FLOCCULATION OF SACCHAROMYCES CEREVISIAE.
  • P. Mill
  • Biology
    Journal of general microbiology
  • 1964
TLDR
The point in the growth cycle at which a strain of brewer's yeast became potentially flocculent could be delayed by supplementing the medium with ammonia, basic amino acids, glutamine, asparagine, γ-aminobutyric acid or urea, and it is suggested that the maintenance of non-flocculence is dependent upon the presence in the cell wall of a nitrogenous compound.
Repression and induction of flocculation interactions in Saccharomyces cerevisiae
TLDR
It is demonstrated that the phenotypic expression of FLO1 could be reproducibly manipulated for experimental purposes by aeration alone and a clear correlation between specific protein bands and flocculence could be established.
Yeast flocculation: Flocculation onset and receptor availability
TLDR
The presence of carbohydrate receptors, outer‐chain mannan side‐branches, was monitored throughout growth of flocculant and non‐flocculent strains of Saccharomyces cerevisiae, with particular attention to the growth phases where flocculation is normally developed.
Genetic aspects of yeast flocculation : in particular, the role of FLO genes in the flocculation of Saccharomyces cerevisiae
Characterization of non-flocculent cells isolated from a culture of flocculent Saccharomyces cerevisiae NCYC 1001.
TLDR
During cultivation of a flocculent yeast, Saccharomyces cerevisiae 1001, two cell fractions, flocs and free cells, appeared in the medium and the culture showed synchronous growth.
Determinants of flocculence of brewer's yeast during fermentation in wort
TLDR
The hypothesis that cell surface hydrophobicity is a major determinant for yeast cells to become flocculent is supported, and it is suggested that shortage of sterols and unsaturated fatty acids precedesFlocculence under brewing conditions.
Population dynamics of flocculating yeasts.
TLDR
Using adhesion experiments, it is demonstrated that cells of non-flocculent strain interact by establishing a true bond with flocculant cells rather than by entrapment inside the floc matrix, indicating that the inhibition of flocculation is due to inhibition or inactivation of 'lectin-like' component.
...
1
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