Flippase activity detected with unlabeled lipids by shape changes of giant unilamellar vesicles.

@article{Papadopulos2007FlippaseAD,
  title={Flippase activity detected with unlabeled lipids by shape changes of giant unilamellar vesicles.},
  author={Andreas Papadopulos and Stefanie Vehring and Iv{\'a}n L{\'o}pez-Montero and Lara Kutschenko and Martin St{\"o}ckl and Philippe F. Devaux and Michael M. Kozlov and Thomas Pomorski and Andreas Herrmann},
  journal={The Journal of biological chemistry},
  year={2007},
  volume={282 21},
  pages={15559-68}
}
Transbilayer movement of phospholipids in biological membranes is mediated by energy-dependent and energy-independent flippases. Available methods for detection of flippase mediated transversal flip-flop are essentially based on spin-labeled or fluorescent lipid analogues. Here we demonstrate that shape change of giant unilamellar vesicles (GUVs) can be used as a new tool to study the occurrence and time scale of flippase-mediated transbilayer movement of unlabeled phospholipids. Insertion of… CONTINUE READING
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