Flexible regions of RNA structure facilitate co-operative Rev assembly on the Rev-response element.

@article{Zemmel1996FlexibleRO,
  title={Flexible regions of RNA structure facilitate co-operative Rev assembly on the Rev-response element.},
  author={Rodney Zemmel and Ann C. Kelley and Jonathan Karn and P. Jonathan G. Butler},
  journal={Journal of molecular biology},
  year={1996},
  volume={258 5},
  pages={763-77}
}
The oligomerisation of Rev on the Rev-response element (RRE) was studied using a series of model substrates. Only a monomer of Rev is able to bind efficiently to a high affinity site that is flanked by perfect duplex RNA. Addition of a bulge or a second stem structure adjacent to the high affinity site permits the co-operative incorporation of a second Rev molecule to the RNA. Model RREs carrying bulges can bind Rev with a higher degree of co-operativity than the native structure… CONTINUE READING