Flexibility, diversity, and cooperativity: pillars of enzyme catalysis.

  title={Flexibility, diversity, and cooperativity: pillars of enzyme catalysis.},
  author={Gordon G. Hammes and Stephen J Benkovic and Sharon Hammes-Schiffer},
  volume={50 48},
This brief review discusses our current understanding of the molecular basis of enzyme catalysis. A historical development is presented, beginning with steady state kinetics and progressing through modern fast reaction methods, nuclear magnetic resonance, and single-molecule fluorescence techniques. Experimental results are summarized for ribonuclease, aspartate aminotransferase, and especially dihydrofolate reductase (DHFR). Multiple intermediates, multiple conformations, and cooperative… CONTINUE READING