Flavonol Synthase Activity and the Regulation of Flavonol and Anthocyanin Biosynthesis during Flower Development in Dianthus caryophyllus L. (Carnation)

Abstract

Flavonol synthase (FLS) was demonstrated in crude extracts from flower buds o f Dianthus caryophyllus (carnation). The enzyme catalyzed the conversion o f dihydrokaempferol and dihydroquercetin to kaempferol and quercetin, respectively. The reaction required 2-oxoglutarate, ferrous ion and ascorbate as co-factors and had a pH optimum at about 7.4. The… (More)

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