Flavonoid-mediated inhibition of SARS coronavirus 3C-like protease expressed in Pichia pastoris
@article{Nguyen2011FlavonoidmediatedIO, title={Flavonoid-mediated inhibition of SARS coronavirus 3C-like protease expressed in Pichia pastoris}, author={Thi Thanh Hanh Nguyen and Hye Jin Woo and Hee‐Kyoung Kang and Van Dao Nguyen and Young-Min Kim and Do-Won Kim and Sul-Ah Ahn and Yongmei Xia and Doman Kim}, journal={Biotechnology Letters}, year={2011}, volume={34}, pages={831 - 838} }
The 3C-like protease (3CLpro) of severe acute respiratory syndrome associated coronavirus (SARS-CoV) is vital for SARS-CoV replication and is a promising drug target. Recombinant 3CLpro was expressed in Pichia pastoris GS115 as a 42 kDa protein that displayed a Km of 15 ± 2 μM with Dabcyl-KTSAVLQSGFRKME-Edans as substrate. Purified 3CLpro was used for inhibition and kinetic assays with seven flavonoid compounds. The IC50 of six flavonoid compounds were 47–381 μM. Quercetin, epigallocatechin…
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