Flavonoid-mediated inhibition of SARS coronavirus 3C-like protease expressed in Pichia pastoris

@article{Nguyen2011FlavonoidmediatedIO,
  title={Flavonoid-mediated inhibition of SARS coronavirus 3C-like protease expressed in Pichia pastoris},
  author={Thi Thanh Hanh Nguyen and Hye-Jin Woo and Hee Kyoung Kang and Van Dao Nguyen and Young-Min Kim and Do Won Kim and Sul-Ah Ahn and Yongmei Xia and Doman Kim},
  journal={Biotechnology Letters},
  year={2011},
  volume={34},
  pages={831-838}
}
The 3C-like protease (3CLpro) of severe acute respiratory syndrome associated coronavirus (SARS-CoV) is vital for SARS-CoV replication and is a promising drug target. Recombinant 3CLpro was expressed in Pichia pastoris GS115 as a 42 kDa protein that displayed a Km of 15 ± 2 μM with Dabcyl-KTSAVLQSGFRKME-Edans as substrate. Purified 3CLpro was used for inhibition and kinetic assays with seven flavonoid compounds. The IC50 of six flavonoid compounds were 47–381 μM. Quercetin, epigallocatechin… CONTINUE READING

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