Flavonoid 3'-O-methyltransferase from rice: cDNA cloning, characterization and functional expression.

@article{Kim2006Flavonoid3F,
  title={Flavonoid 3'-O-methyltransferase from rice: cDNA cloning, characterization and functional expression.},
  author={Bong-Gyu Kim and Youngshim Lee and Hor-Gil Hur and Yoongho Lim and J H Ahn},
  journal={Phytochemistry},
  year={2006},
  volume={67 4},
  pages={387-94}
}
Plant O-methyltransferases (OMTs) are known to be involved in methylation of plant secondary metabolites, especially phenylpropanoid and flavonoid compounds. An OMT, ROMT-9, was cloned and characterized from rice using a reverse transcriptase polymerase chain reaction (RT-PCR). The blast results for ROMT-9 showed a 73% identity with caffeic acid OMTs from maize and Triticum aestivum. ROMT-9 was expressed in Escherichia coli and its recombinant protein was purified using affinity chromatography… CONTINUE READING
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