Flavokinase and FAD synthetase from Bacillus subtilis specific for reduced flavins.

@article{Kearney1979FlavokinaseAF,
  title={Flavokinase and FAD synthetase from Bacillus subtilis specific for reduced flavins.},
  author={Edna B. Kearney and Jos{\'e} Goldenberg and Joseph S Lipsick and Meir Perl},
  journal={The Journal of biological chemistry},
  year={1979},
  volume={254 19},
  pages={9551-7}
}
A flavokinase preparation from Bacillus subtilis is described which catalyzes the phosphorylation of reduced, but not oxidized, riboflavin. The enzyme is distinguished from other known flavokinases also in having an unusually low Km for the flavin substrate, 50 to 100 nM. ATP is the obligatory phosphate donor; one ATP is utilized for each FMNH2 formed. Mg2… CONTINUE READING