Flattening and/or expanding of pH gradients in isoelectric focusing gels exemplified with PhastSystem.

@article{Hackler1988FlatteningAE,
  title={Flattening and/or expanding of pH gradients in isoelectric focusing gels exemplified with PhastSystem.},
  author={Roif Hackler and T. O. Kleine},
  journal={Electrophoresis},
  year={1988},
  volume={9 6},
  pages={262-7}
}
A simple method of flattening and/or expanding of pH gradients in isoelectric focusing is described for any pH interval desired: to modify pH gradients near one electrode a paper strip soaked with carrier ampholytes is applied onto the gel close to the opposite electrode. In order to flatten central parts of pH intervals paper strips are applied onto the… CONTINUE READING