Flagellin gene (fliC) of Thermus thermophilus HB8: characterization of its product and involvement to flagella assembly and microbial motility

@article{Papaneophytou2012FlagellinG,
  title={Flagellin gene (fliC) of Thermus thermophilus HB8: characterization of its product and involvement to flagella assembly and microbial motility},
  author={Christos P. Papaneophytou and Rigini M. Papi and Anastasia A. Pantazaki and Dimitrios A. Kyriakidis},
  journal={Applied Microbiology and Biotechnology},
  year={2012},
  volume={94},
  pages={1265-1277}
}
Thermus thermophilus HB8 flagellin protein (FliC) is encoded by the TTHC004 (fliC) gene, which is located in the pTT8 plasmid of the bacterium. Flagellin monomer and flagella fibres were isolated from a culture of T. thermophilus grown in rich medium, or in mineral salt medium with sodium gluconate as the carbon source. Western blot immunodetection with anti-FliC revealed a stable complex (FliC)1(FliS)2 of flagellin (FliC, 27.7 kDa) with a homodimer of FliS (FliS, 18.2 kDa) that are encoded by… CONTINUE READING

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