Fixed-target protein serial microcrystallography with an x-ray free electron laser

  title={Fixed-target protein serial microcrystallography with an x-ray free electron laser},
  author={Mark S Hunter and Brent W. Segelke and Marc Messerschmidt and Garth Brokaw RoseMarie Fagan Cele Jackson Carter C. Williams and Nadia A. Zatsepin and Anton Barty and William Henry Benner and David B. Carlson and Matthew A. Coleman and Alexander Graf and Stefan P. Hau-Riege and Tommaso Pardini and M. Marvin Seibert and James E Evans and S{\'e}bastien Boutet and Matthias Frank},
  booktitle={Scientific reports},
We present results from experiments at the Linac Coherent Light Source (LCLS) demonstrating that serial femtosecond crystallography (SFX) can be performed to high resolution (~2.5 Å) using protein microcrystals deposited on an ultra-thin silicon nitride membrane and embedded in a preservation medium at room temperature. Data can be acquired at a high acquisition rate using x-ray free electron laser sources to overcome radiation damage, while sample consumption is dramatically reduced compared… CONTINUE READING
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