• DOI:10.1038/nmeth.3629
Corpus ID: 14548269

Fixed single-cell transcriptomic characterization of human radial glial diversity

@article{Thomsen2016FixedST,
  title={Fixed single-cell transcriptomic characterization of human radial glial diversity},
  author={Elliot R. Thomsen and John K. Mich and Zizhen Yao and R. Hodge and Adele M Doyle and Sumin Jang and Soraya I. Shehata and A. Nelson and Nadiya Shapovalova and B. Levi and Sharad Ramanathan},
  journal={Nature methods},
  year={2016},
  volume={13},
  pages={87 - 93}
}

Elliot R. Thomsen, John K. Mich, +8 authors Sharad Ramanathan

Published 2016

Biology, Medicine

Nature methods

The diverse progenitors that give rise to the human neocortex have been difficult to characterize because progenitors, particularly radial glia (RG), are rare and are defined by a combination of intracellular markers, position and morphology. To circumvent these problems, we developed Fixed and Recovered Intact Single-cell RNA (FRISCR), a method for profiling the transcriptomes of individual fixed, stained and sorted cells. Using FRISCR, we profiled primary human RG that constitute only 1% of… CONTINUE READING

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