Fixed single-cell transcriptomic characterization of human radial glial diversity

  title={Fixed single-cell transcriptomic characterization of human radial glial diversity},
  author={Elliot R. Thomsen and John K. Mich and Zizhen Yao and R. Hodge and Adele M Doyle and Sumin Jang and Soraya I. Shehata and A. Nelson and Nadiya Shapovalova and B. Levi and Sharad Ramanathan},
  journal={Nature methods},
  pages={87 - 93}
  • Elliot R. Thomsen, John K. Mich, +8 authors Sharad Ramanathan
  • Published 2016
  • Biology, Medicine
  • Nature methods
  • The diverse progenitors that give rise to the human neocortex have been difficult to characterize because progenitors, particularly radial glia (RG), are rare and are defined by a combination of intracellular markers, position and morphology. To circumvent these problems, we developed Fixed and Recovered Intact Single-cell RNA (FRISCR), a method for profiling the transcriptomes of individual fixed, stained and sorted cells. Using FRISCR, we profiled primary human RG that constitute only 1% of… CONTINUE READING
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