Five phosphonate operon gene products as components of a multi-subunit complex of the carbon-phosphorus lyase pathway.

@article{Jochimsen2011FivePO,
  title={Five phosphonate operon gene products as components of a multi-subunit complex of the carbon-phosphorus lyase pathway.},
  author={Bjarne U. Jochimsen and Signe Lolle and Fern R. McSorley and Mariah Nabi and Jens Stougaard and David L. Zechel and Bjarne Hove-Jensen},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2011},
  volume={108 28},
  pages={11393-8}
}
Organophosphonate utilization by Escherichia coli requires the 14 cistrons of the phnCDEFGHIJKLMNOP operon, of which the carbon-phosphorus lyase has been postulated to consist of the seven polypeptides specified by phnG to phnM. A 5,660-bp DNA fragment encompassing phnGHIJKLM is cloned, followed by expression in E. coli and purification of Phn-polypeptides. PhnG, PhnH, PhnI, PhnJ, and PhnK copurify as a protein complex by ion-exchange, size-exclusion, and affinity chromatography. The five… CONTINUE READING
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Crystal structure of PhnH: An essential component of carbonphosphorus lyase in Escherichia coli

  • MA Adams
  • J Bacteriol
  • 2008

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