Five percent of normal cystic fibrosis transmembrane conductance regulator mRNA ameliorates the severity of pulmonary disease in cystic fibrosis.

@article{Ramalho2002FivePO,
  title={Five percent of normal cystic fibrosis transmembrane conductance regulator mRNA ameliorates the severity of pulmonary disease in cystic fibrosis.},
  author={Anabela Santo Ramalho and Sebastian Beck and Michelle E Meyer and Deborah Penque and Garry R Cutting and Margarida D. Amaral},
  journal={American journal of respiratory cell and molecular biology},
  year={2002},
  volume={27 5},
  pages={
          619-27
        }
}
  • A. Ramalho, S. Beck, M. Amaral
  • Published 1 November 2002
  • Biology, Medicine
  • American journal of respiratory cell and molecular biology
Estimates of the level of transcripts from the cystic fibrosis (CF) transmembrane conductance regulator (CFTR) gene required to develop a CF phenotype range from 4-20% of normal. Due to the importance of obtaining reliable data on this issue for therapeutic strategies, we developed a novel polymerase chain reaction-based method to quantify CFTR transcripts and applied it to the analysis of nasal epithelium RNA of five patients with CF and the 3272-26A>G/F508del genotype. We calculated that 8.2… 
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