Five percent of normal cystic fibrosis transmembrane conductance regulator mRNA ameliorates the severity of pulmonary disease in cystic fibrosis.
@article{Ramalho2002FivePO, title={Five percent of normal cystic fibrosis transmembrane conductance regulator mRNA ameliorates the severity of pulmonary disease in cystic fibrosis.}, author={Anabela Santo Ramalho and Sebastian Beck and Michelle E Meyer and Deborah Penque and Garry R. Cutting and Margarida D. Amaral}, journal={American journal of respiratory cell and molecular biology}, year={2002}, volume={27 5}, pages={ 619-27 } }
Estimates of the level of transcripts from the cystic fibrosis (CF) transmembrane conductance regulator (CFTR) gene required to develop a CF phenotype range from 4-20% of normal. Due to the importance of obtaining reliable data on this issue for therapeutic strategies, we developed a novel polymerase chain reaction-based method to quantify CFTR transcripts and applied it to the analysis of nasal epithelium RNA of five patients with CF and the 3272-26A>G/F508del genotype. We calculated that 8.2…
182 Citations
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